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nbp2 33524  (Novus Biologicals)


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    Novus Biologicals nbp2 33524
    Nbp2 33524, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nbp2 33524/product/Novus Biologicals
    Average 90 stars, based on 2 article reviews
    nbp2 33524 - by Bioz Stars, 2026-03
    90/100 stars

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    Among 22 normal tissues (see ), ADAM2, <t>CALR3,</t> SAGE1 and MAGE-A proteins were only detected in the germ cells of the testis. More specifically, SAGE-1 and MAGE-A were found in spermatogonia, while ADAM2 and CALR3 were present in post-meiotic germ cells. Only MAGE-A proteins were detected in plancenta (all pictures magnification x20).
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    Among 22 normal tissues (see ), ADAM2, CALR3, SAGE1 and MAGE-A proteins were only detected in the germ cells of the testis. More specifically, SAGE-1 and MAGE-A were found in spermatogonia, while ADAM2 and CALR3 were present in post-meiotic germ cells. Only MAGE-A proteins were detected in plancenta (all pictures magnification x20).

    Journal: PLoS ONE

    Article Title: Lack of ADAM2, CALR3 and SAGE1 Cancer/Testis Antigen Expression in Lung and Breast Cancer

    doi: 10.1371/journal.pone.0134967

    Figure Lengend Snippet: Among 22 normal tissues (see ), ADAM2, CALR3, SAGE1 and MAGE-A proteins were only detected in the germ cells of the testis. More specifically, SAGE-1 and MAGE-A were found in spermatogonia, while ADAM2 and CALR3 were present in post-meiotic germ cells. Only MAGE-A proteins were detected in plancenta (all pictures magnification x20).

    Article Snippet: To optimize conditions for staining with antibodies raised against amino acids 122–215 of ADAM2 (rabbit polyclonal, HPA026581; Sigma Aldrich, Brøndby), amino acids 195–294 of CALR3 (rabbit polyclonal, NBP2-33524, Novus, Littleton, CO, USA) and amino acids 497–641 of SAGE1 (rabbit polyclonal, HPA003208, Sigma Aldrich), different concentrations were tested in combination with different antigen retrieval protocols using sections of human testis.

    Techniques:

    ADAM2, CALR3, SAGE1 and MAGE-A expression in lung cancer.

    Journal: PLoS ONE

    Article Title: Lack of ADAM2, CALR3 and SAGE1 Cancer/Testis Antigen Expression in Lung and Breast Cancer

    doi: 10.1371/journal.pone.0134967

    Figure Lengend Snippet: ADAM2, CALR3, SAGE1 and MAGE-A expression in lung cancer.

    Article Snippet: To optimize conditions for staining with antibodies raised against amino acids 122–215 of ADAM2 (rabbit polyclonal, HPA026581; Sigma Aldrich, Brøndby), amino acids 195–294 of CALR3 (rabbit polyclonal, NBP2-33524, Novus, Littleton, CO, USA) and amino acids 497–641 of SAGE1 (rabbit polyclonal, HPA003208, Sigma Aldrich), different concentrations were tested in combination with different antigen retrieval protocols using sections of human testis.

    Techniques: Expressing

    Examples of lung and breast cancer tumors positive for MAGE-A and negative for ADAM2, CALR3 and SAGE1 (all pictures magnification x20).

    Journal: PLoS ONE

    Article Title: Lack of ADAM2, CALR3 and SAGE1 Cancer/Testis Antigen Expression in Lung and Breast Cancer

    doi: 10.1371/journal.pone.0134967

    Figure Lengend Snippet: Examples of lung and breast cancer tumors positive for MAGE-A and negative for ADAM2, CALR3 and SAGE1 (all pictures magnification x20).

    Article Snippet: To optimize conditions for staining with antibodies raised against amino acids 122–215 of ADAM2 (rabbit polyclonal, HPA026581; Sigma Aldrich, Brøndby), amino acids 195–294 of CALR3 (rabbit polyclonal, NBP2-33524, Novus, Littleton, CO, USA) and amino acids 497–641 of SAGE1 (rabbit polyclonal, HPA003208, Sigma Aldrich), different concentrations were tested in combination with different antigen retrieval protocols using sections of human testis.

    Techniques:

    Analysis of ADAM2, CALR3, SAGE1 and MAGE-A expression in cohort 1 consistent of 68 breast cancers of different clinical stages.

    Journal: PLoS ONE

    Article Title: Lack of ADAM2, CALR3 and SAGE1 Cancer/Testis Antigen Expression in Lung and Breast Cancer

    doi: 10.1371/journal.pone.0134967

    Figure Lengend Snippet: Analysis of ADAM2, CALR3, SAGE1 and MAGE-A expression in cohort 1 consistent of 68 breast cancers of different clinical stages.

    Article Snippet: To optimize conditions for staining with antibodies raised against amino acids 122–215 of ADAM2 (rabbit polyclonal, HPA026581; Sigma Aldrich, Brøndby), amino acids 195–294 of CALR3 (rabbit polyclonal, NBP2-33524, Novus, Littleton, CO, USA) and amino acids 497–641 of SAGE1 (rabbit polyclonal, HPA003208, Sigma Aldrich), different concentrations were tested in combination with different antigen retrieval protocols using sections of human testis.

    Techniques: Expressing

    Analysis of ADAM2, CALR3 and MAGE-A expression in cohort 2 consisting of 121 breast cancers of different receptor status.

    Journal: PLoS ONE

    Article Title: Lack of ADAM2, CALR3 and SAGE1 Cancer/Testis Antigen Expression in Lung and Breast Cancer

    doi: 10.1371/journal.pone.0134967

    Figure Lengend Snippet: Analysis of ADAM2, CALR3 and MAGE-A expression in cohort 2 consisting of 121 breast cancers of different receptor status.

    Article Snippet: To optimize conditions for staining with antibodies raised against amino acids 122–215 of ADAM2 (rabbit polyclonal, HPA026581; Sigma Aldrich, Brøndby), amino acids 195–294 of CALR3 (rabbit polyclonal, NBP2-33524, Novus, Littleton, CO, USA) and amino acids 497–641 of SAGE1 (rabbit polyclonal, HPA003208, Sigma Aldrich), different concentrations were tested in combination with different antigen retrieval protocols using sections of human testis.

    Techniques: Expressing

    Expression of MAGE-A1, ADAM2, CALR3 and SAGE1 CT antigen genes in 5-aza-2’-deoxycytidine-treated (5 μM, 48 hours) and untreated lung (A) and breast (B) cancer cell lines was measured using quantitative PCR. Error bars = standard deviation.

    Journal: PLoS ONE

    Article Title: Lack of ADAM2, CALR3 and SAGE1 Cancer/Testis Antigen Expression in Lung and Breast Cancer

    doi: 10.1371/journal.pone.0134967

    Figure Lengend Snippet: Expression of MAGE-A1, ADAM2, CALR3 and SAGE1 CT antigen genes in 5-aza-2’-deoxycytidine-treated (5 μM, 48 hours) and untreated lung (A) and breast (B) cancer cell lines was measured using quantitative PCR. Error bars = standard deviation.

    Article Snippet: To optimize conditions for staining with antibodies raised against amino acids 122–215 of ADAM2 (rabbit polyclonal, HPA026581; Sigma Aldrich, Brøndby), amino acids 195–294 of CALR3 (rabbit polyclonal, NBP2-33524, Novus, Littleton, CO, USA) and amino acids 497–641 of SAGE1 (rabbit polyclonal, HPA003208, Sigma Aldrich), different concentrations were tested in combination with different antigen retrieval protocols using sections of human testis.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Standard Deviation